种属

人

别称

SNF96.2; sNF96-2

组织来源

施万细胞

疾病

1型神经纤维瘤病Nf1

传代比例/细胞消化

1:2传代,

完全培养基配置

DMEM培养基 ；10%胎牛血清 ；1%双抗

简介

sNF96.2 is a Schwann cell line that was isolated in 1996 from a 28-year-old, male patient with neurofibromatosis type 1 Nf1.The sNF96.2 cell line was derived from a recurrent mass associated with nerve and diagnosed as MPNST (Malignant Peripheral Nerve Sheath Tumor) in a patient meeting NF1 diagnostic criteria. The cells were derived from numerous passages of primary tumor material in culture until they were a homogenous Schwann cell-like population which displayed a clonal morphology immunopositive for both cytoplasmic Schwann cell markers S100 and p75.

形态

雪旺细胞样

生长特征

   贴壁生长

STR

Amelogenin X CSF1PO 12 D5S818 11 D7S820 10,11 D13S317 10 D16S539 11 TH01 6 TPOX 11

vWA 17,19

倍增时间

~33 h

培养条件

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。

冻存条件

冻存液：90%FBS，DMSO 10%,

或使用非程序冻存液：官网货号JY-H040

保藏机构  

ATCC; CRL-2884

产品使用

仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。