|
种属 |
人 |
|
别称 |
SK-Mel-5; SK MEL 5; SK.MEL.5; SK-MEL5; SKMel-5; SKMEL-5; SKMEL5; SKMel5; SKmel5; AA-Mel |
|
组织来源 |
皮肤 |
|
疾病 |
皮肤黑色素瘤 |
|
传代比例/细胞消化 |
1:2传代,消化2-3分钟 |
|
完全培养基配置 |
MEM培养基；10%胎牛血清；1%双抗 |
|
简介 |
SK-MEL-5 is a melanoma cell line isolated from skin tissue obtained from a 24-year-old, White, female malignant melanoma patient. This cell line is a suitable transfection host and also serves as a source of target cells for the detection of melanoma-specific antibody in patients with the disease. This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates. |
|
形态 |
星状细胞样 |
|
生长特征 |
贴壁生长 |
|
倍增时间 |
每周 2 至 3 次 |
|
抗原表达 |
Blood Type O; Rh+; HLA A2, A11, B40, Bw16 |
|
致瘤性 |
Yes, in nude mice; forms malignant melanoma |
|
STR |
Amelogenin: X CSF1PO: 10,13 D13S317: 10,12 D16S539: 10,12 D5S818: 11,13 D7S820: 9,12 THO1: 6,9 TPOX: 11 vWA: 14,18 |
|
培养条件 |
气相：空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。 |
|
冻存条件 |
冻存液：90%FBS，DMSO 10%, 或使用非程序冻存液：官网货号JY-H040 |
|
保藏机构 |
ATCC; HTB-70 |
|
产品使用 |
仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。 |